abstract：:A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...

journal_title：Microbiology and immunology

authors： Kitamura-Muramatsu Y,Kusumoto-Matsuo R,Kondo K,Mori S,Saito S,Tsukahara Y,Kukimoto I

更新日期：2012-02-01 00:00:00

abstract：:The genus Enhydrobacter, first reported as a member of the family Vibrionaceae, has been placed in the family Moraxellaceae, but as a genus incertae sedis in Bergey's Manual of Systematic Bacteriology 2nd edition. During our taxonomic investigation of Enhydrobacter-like organisms, we observed that the 16S rRNA sequenc...

journal_title：Microbiology and immunology

authors： Kawamura Y,Fujiwara N,Naka T,Mitani A,Kubota H,Tomida J,Morita Y,Hitomi J

更新日期：2012-01-01 00:00:00

abstract：:Chronic hepatitis C virus (HCV) infection affects approximately 170 million people worldwide. HCV infection is a major global health problem as it can be complicated with liver cirrhosis and hepatocellular carcinoma. So far, there is no vaccine available and the non-specific, interferon (IFN)-based treatments now in u...

journal_title：Microbiology and immunology

authors： Aly HH,Shimotohno K,Hijikata M,Seya T

更新日期：2012-01-01 00:00:00

abstract：:Triosephosphate isomerase (TPI; EC 5. 3. 1. 1) displayed on the cell surface of Staphylococcus aureus acts as an adhesion molecule that binds to the capsule of Cryptococcus neoformans, a fungal pathogen. This study investigated the function of TPI on the cell surface of S. aureus and its interactions with biological s...

journal_title：Microbiology and immunology

authors： Furuya H,Ikeda R

更新日期：2011-12-01 00:00:00

abstract：:In innate immunity, cationic antimicrobial peptides including cathelin-related antimicrobial peptide (CRAMP) are known to play critical roles in protecting the host from infection by invasive microbes, including Gram-positive and -negative bacteria. However, little is known about the interactions between CRAMP and myc...

journal_title：Microbiology and immunology

authors： Tani K,Shimizu T,Kida Y,Kuwano K

更新日期：2011-08-01 00:00:00

abstract：:Despite the high prevalence of highly pathogenic H5N1 influenza A viruses in Indonesia, epidemiology information on seasonal human influenza is lacking. The present authors, therefore, conducted virologic surveillance in Surabaya, East Java from October 2008 to March 2010. Influenza viruses, including pandemic (H1N1) ...

journal_title：Microbiology and immunology

authors： Yamaoka M,Palilingan JF,Wibisono J,Yudhawati R,Nidom RV,Alamudi MY,Ginting TE,Makino A,Nidom CA,Shinya K,Kawaoka Y

更新日期：2011-07-01 00:00:00

abstract：:A novel combination of antimicrobial agents (sulfamethoxazole, 40 μg/mL; trimethoprim, 20 μg/mL; amphotericin B, 5 μg/mL; fosfomycin, 400 μg/mL; and 5-fluorouracil, 100 μg/mL) was developed for selective isolation of leptospires from contaminated samples. The growth of 16 microorganisms considered as possible contamin...

journal_title：Microbiology and immunology

authors： Chakraborty A,Miyahara S,Villanueva SY,Saito M,Gloriani NG,Yoshida S

更新日期：2011-07-01 00:00:00

abstract：:M protein is an important virulence determinant in Streptococcus pyogenes, but the amounts of M protein in various strains of the species remain to be elucidated. To assess the amount of M protein in strains of each emm genotype, dot blot analysis was performed on 141 clinically isolated strains. Among the cell membra...

journal_title：Microbiology and immunology

authors： Matsumoto M,Suzuki M,Hirose K,Hiramatsu R,Minagawa H,Minami M,Tatsuno I,Okamoto A,Ohta M,Hasegawa T

更新日期：2011-06-01 00:00:00

abstract：:Recipient cells migrating into the transplantation site of an allograft recognize histocompatibility antigens on the grafts and are cytotoxic against the grafts. Although the alloreactive immune response is predominantly directed at the major histocompatibility complex (major histocompatibility complex [MHC]; H-2 in m...

journal_title：Microbiology and immunology

authors： Inoue Y,Tashiro-Yamaji J,Hayashi M,Kiyonari H,Shimizu T,Ibata M,Yamana H,Kubota T,Tanigawa N,Yoshida R

更新日期：2011-06-01 00:00:00

abstract：:A chimeric PCV1-2 clone containing the PCV2 capsid gene cloned into the backbone of the nonpathogenic PCV1 genome was recently generated based on PCV2 and PCV1 strains isolated in China. The efficacy of this available candidate inactivated vaccine was evaluated by subjecting conventional pigs to intramuscular immuniza...

journal_title：Microbiology and immunology

authors： Xujie L,Xiaobo W,Yi S,Jing F,Song G,Xiufan L

更新日期：2011-04-01 00:00:00

abstract：:Unmethylated CpG-ODN are known to enhance Th1-type immune response. However, optimal sequences of CpG-ODN for activating Th1-type immune cells vary among species. It is necessary to identify the effective CpG-ODN sequences in each species. In the present study, in order to identify the sequences of CpG-ODN that produc...

journal_title：Microbiology and immunology

authors： Satoh R,Kotake M,Takano T,Motokawa K,Gemma T,Watanabe R,Arai S,Hohdatsu T

更新日期：2011-03-01 00:00:00

abstract：:The current vaccination strategy against influenza is to induce production of antibodies directed against the surface antigens of these viruses. However, frequent changes in the surface antigens of influenza viruses allow them to avoid antibody-mediated immunity. On the other hand, it is known that cytotoxic T-lymphoc...

journal_title：Microbiology and immunology

authors： Uchida T

更新日期：2011-01-01 00:00:00

abstract：:β-1,3-glucan is a major component of fungal cell walls with various biological activities, including effects on the production of inflammatory mediators in vivo and in vitro. However, few reports have examined its influence on the fungal cell itself. In this study, the influences of β-1,3-glucan on the growth and cell...

journal_title：Microbiology and immunology

authors： Ishibashi K,Kurone Y,Motoi M,Miura NN,Adachi Y,Shirasu Y,Ohno N

更新日期：2010-11-01 00:00:00

abstract：:Our objective was to study the alterations of CD4(+) CD25(+) Foxp3(+) T(regs) in HIV-infected SPs and to examine the role of T(regs) in the disease progression of HIV. The proportion of CD4(+) CD25(+) Foxp3(+) T(regs) in peripheral blood of 24 SPs, 30 asymptomatic HIV-infected patients, 20 AIDS patients, and 16 non-in...

journal_title：Microbiology and immunology

authors： Zhang Z,Jiang Y,Zhang M,Liu J,Sun G,Shi W,Wang Y,Shang H

更新日期：2010-10-01 00:00:00

abstract：:Quantitative PCR is becoming widespread for diagnosing and monitoring post-transplantation diseases associated with EBV and CMV. These assays need to be standardized to manage patients in different facilities. Five independent laboratories in Japan compared home-brew assays and a prototype assay system to establish a ...

journal_title：Microbiology and immunology

authors： Ito Y,Takakura S,Ichiyama S,Ueda M,Ando Y,Matsuda K,Hidaka E,Nakatani K,Nishioka J,Nobori T,Kajiyama N,Kimura H

更新日期：2010-09-01 00:00:00

abstract：:In the present study, 12 patients with fever of undetermined origin, anemia and icterus were diagnosed with hemoplasma infection by light microscopy, transmission electron microscopy examination and PCR assay after being excluded from other usual febrile diseases. Complement receptor type I (CR1, CD35) expression on t...

journal_title：Microbiology and immunology

authors： Congbin Y,Aibin L,Congli Y,Juan X,Lanjun B,Weiping Z,Zhibiao Y,Xiuguo H

更新日期：2010-08-01 00:00:00

abstract：:EAEC is increasingly recognized as an emerging enteric pathogen. Typical EAEC expressing the AggR regulon have been proven to be an important cause of childhood diarrhea in industrialized countries as well as in the developing world, while atypical EAEC without this regulon have not been thoroughly investigated. To in...

journal_title：Microbiology and immunology

authors： Tokuda K,Nishi J,Imuta N,Fujiyama R,Kamenosono A,Manago K,Kawano Y

更新日期：2010-06-01 00:00:00

abstract：:The Clostridium perfringens strain 13 genome contains two genes (fbpA, fbpB) that encode putative Fbp. Both rFbpA and rFbpB were purified and their reactivity with human serum Fn was analyzed. To determine the region of the Fn molecule recognized by rFbp, a plate binding assay using N-terminal 70-kDa peptide, III1-C p...

journal_title：Microbiology and immunology

authors： Yamasaki T,Hitsumoto Y,Katayama S,Nogami Y

更新日期：2010-04-01 00:00:00

abstract：:The effects of the soluble forms of the endotoxin receptor molecules sMD-2 and sCD14 on bacterial growth were studied. When Escherichia coli and Bacillus subtilis were incubated at 37 degrees C for 18 hr with either sMD-2 or sCD14, growth of these bacteria was significantly inhibited as evaluated by viable cell counts...

journal_title：Microbiology and immunology

authors： Ohnishi T,Muroi M,Tanamoto K

更新日期：2010-02-01 00:00:00

abstract：:The extract from ECMS was investigated for its effect on the humoral immune responses to foot-and-mouth disease vaccination. Fifty-six mice were randomly divided into seven groups with eight animals in each. Mice in groups 5 to 7 were subcutaneously (s.c.) injected with 0.5 mg DEX daily for 4 days to induce immunosupp...

journal_title：Microbiology and immunology

authors： Sakwiwatkul K,Li RL,Song XM,Hu SH

更新日期：2010-01-01 00:00:00

abstract：:There is a deficiency in the data concerning the role of hMPV in lower respiratory tract infections in adults, and until now there has been no data available regarding the prevalence of hMPV in adults in our region. In the present study the association of hMPV with varieties of lower respiratory tract disorders in imm...

journal_title：Microbiology and immunology

authors： El Sayed Zaki M,Raafat D,El-Metaal AA,Ismail M

更新日期：2009-11-01 00:00:00

abstract：:The effects of LV in two different species, CD-1 mice, without a genetic disposition for diabetes, and BB rats prone to T1D were examined. Male CD-1 mice that had been exposed to LV in utero developed a type 2-like diabetes with increased blood glucose, insulin levels and epididymal fat at the age of 10-15 weeks. Comb...

journal_title：Microbiology and immunology

authors： Holmberg R,Klitz W,Blixt M,Berggren PO,Juntti-Berggren L,Niklasson B

更新日期：2009-10-01 00:00:00

abstract：:A number of studies have determined the contribution of Th1 and Th2 responses to the protective immunity and pathology of Mycobacterium bovis infection. However, much of that information is derived from experimentally infecting cattle with M. bovis and few data from naturally infected animals are available. The aim of...

journal_title：Microbiology and immunology

authors： Blanco FC,Schierloh P,Bianco MV,Caimi K,Meikle V,Alito AE,Cataldi AA,Sasiain Mdel C,Bigi F

更新日期：2009-08-01 00:00:00

abstract：:The gH of CMV is a major target for strain-specific neutralizing antibodies. To verify whether there is a correlation between HLA-DR type and strain-specific antibodies, antibodies against CMV gH in potential donors and recipients for renal transplantation were investigated. Among 471 subjects, 404 (86%) showed reacti...

journal_title：Microbiology and immunology

authors： Ishibashi K,Tokumoto T,Shirakawa H,Hashimoto K,Kushida N,Yanagida T,Shishido K,Aikawa K,Yamaguchi O,Toma H,Tanabe K,Suzutani T

更新日期：2009-07-01 00:00:00

abstract：:Farnesol is well known as a quorum-sensing molecule of Candida albicans. To assess the pathological function of farnesol, its effects on macrophage viability and functions including growth inhibitory activities against C. albicans were examined in vitro. Murine macrophages, when cultured in the presence of 56-112 micr...

journal_title：Microbiology and immunology

authors： Abe S,Tsunashima R,Iijima R,Yamada T,Maruyama N,Hisajima T,Abe Y,Oshima H,Yamazaki M

更新日期：2009-06-01 00:00:00

abstract：:Periapical lesions are induced by bacterial infection of the dental pulp and result in destruction of the surrounding alveolar bone. Although various immunological studies concerning periapical bone resorption have been reported, the role of cytokines in the formation of periapical lesions remains unclear. In this stu...

journal_title：Microbiology and immunology

authors： Oseko F,Yamamoto T,Akamatsu Y,Kanamura N,Iwakura Y,Imanishi J,Kita M

更新日期：2009-05-01 00:00:00

abstract：:Proteus mirabilis is commonly associated with complicated UTI and expresses several virulence factors, including MR/P fimbriae. In the present study mice were immunised nasally with MrpA, the structural subunit of MR/P, with or without CT as a mucosal adjuvant. The animals were then challenged with P. mirabilis and in...

journal_title：Microbiology and immunology

authors： Scavone P,Rial A,Umpierrez A,Chabalgoity A,Zunino P

更新日期：2009-04-01 00:00:00

abstract：:We evaluated the efficacy of three SARS vaccine candidates in a murine SARS model utilizing low-virulence Pp and SARS-CoV coinfection. Vaccinated mice were protected from severe respiratory disease in parallel with a low virus titer in the lungs and a high neutralizing antibody titer in the plasma. Importantly, the ad...

journal_title：Microbiology and immunology

authors： Ishii K,Hasegawa H,Nagata N,Ami Y,Fukushi S,Taguchi F,Tsunetsugu-Yokota Y

更新日期：2009-02-01 00:00:00

abstract：:Importance of the overexpression of AcrAB efflux pumps in the low-level resistance of pathogens to antimicrobials requires a better understanding of the AcrAB regulation. The goal of the present research was to study the transcription of acrAB, as well as the genes that play a role in its regulation in Salmonella ente...

journal_title：Microbiology and immunology

authors： Hartog E,Ben-Shalom L,Shachar D,Matthews KR,Yaron S

更新日期：2008-12-01 00:00:00

abstract：:Two methods of TSS diagnosis were evaluated: comparison of symptoms with clinical criteria and monitoring for evidence of selective activation of Vbeta2(+) T cells by the causative toxin, TSS toxin-1 (TSST-1). Ten patients with acute and systemic febrile infections caused by Staphylococcus aureus were monitored for in...

journal_title：Microbiology and immunology

authors： Matsuda Y,Kato H,Ono E,Kikuchi K,Muraoka M,Takagi K,Imanishi K,Itoh S,Itoh T,Ogawa T,Nitta K,Inokuchi S,Hibi T,Ohta H,Uchiyama T

更新日期：2008-11-01 00:00:00

abstract：:The purpose of this study was to clarify the taxonomic relationship between all the serovars and species of the genus Erysipelothrix by performing DNA-DNA hybridization experiments, the customary criterion for separation of bacterial genospecies. A total of 93 strains were isolated from a wide variety of sources, incl...

journal_title：Microbiology and immunology

authors： Takahashi T,Fujisawa T,Umeno A,Kozasa T,Yamamoto K,Sawada T

更新日期：2008-10-01 00:00:00

abstract：:The ompA gene, 981 bp in size and identified in the Mycobacterium bovis genome, was cloned and sequenced. Predicted amino acid sequences showed a protein of molecular weight 33.5 kDa with an isoelectric point of 7.28 and an OmpA conserved domain at the carboxy terminal with signal sequence at the N-terminal. Homology ...

journal_title：Microbiology and immunology

authors： Singh JP,Verma R

更新日期：2008-08-01 00:00:00

abstract：:The Env and Gag proteins of HIV-1 are the two major structural proteins of this retrovirus. The interactions between Env and Gag proteins and their regulation in HIV-1 are required for several steps of the replication cycle, involving not only virus assembly, specifically Env incorporation, but also entry steps after ...

journal_title：Microbiology and immunology

authors： Murakami T

更新日期：2008-05-01 00:00:00

abstract：:To evaluate a modified rapid ELISA method for detecting CAg during Toxoplasma gondii infection, we analyzed the specificity and sensitivity of the ELISA method by using experimental Toxoplasma infection in rabbits and also tested this method in human samples including 5428 serum, 548 cerebrospinal fluid and two breast...

journal_title：Microbiology and immunology

authors： Chen R,Lu S,Lou D,Lin A,Zeng X,Ding Z,Wen L,Ohta N,Wang J,Fu C

更新日期：2008-03-01 00:00:00

abstract：:B. pertussis is a causative agent of whooping cough (pertussis) in humans. Despite wide-scale vaccination in many countries, there is serious concern about pertussis as a re-emerging disease. Re-emergence of pertussis may be explained by several factors: the short duration of protection by the currently available acel...

journal_title：Microbiology and immunology

authors： Abe A,Nagamatsu K,Watanabe M

更新日期：2008-02-01 00:00:00

abstract：:ISG20 is an ribonuclease specific for single-stranded RNA and considered to play a role in innate immunity against virus infections. We herein show that both poly IC, an authentic double-stranded RNA, and IFN-gamma induced ISG20 expression in cultured HUVEC. Poly IC-induced ISG20 expression was inhibited by LY294002, ...

journal_title：Microbiology and immunology

authors： Imaizumi T,Mechti N,Matsumiya T,Sakaki H,Kubota K,Yoshida H,Kimura H,Satoh K

更新日期：2008-01-01 00:00:00

abstract：:Many pathogens colonize host tissues by binding to the extracellular matrix via their cell surface adhesion molecules, which are called MSCRAMMs (microbial surface components recognizing adhesive matrix molecules). Staphylococcus aureus expresses several of these adhesion molecules, some of which bind to fibronectin. ...

journal_title：Microbiology and immunology

authors： Shinji H,Kamada M,Seki K,Tajima A,Iwase T,Masuda S

更新日期：2007-01-01 00:00:00

abstract：:In malignant B lymphoma cells interleukin-10 (IL-10) expression is frequently upregulated. This effect is thought to support to the malignant transformation of these cells and to be a potential target for pharmacotherapy. To define better the mechanism for upregulation of the IL-10 gene, we tested the association betw...

journal_title：Microbiology and immunology

authors： Horie K,Ohashi M,Satoh Y,Sairenji T

更新日期：2007-01-01 00:00:00

abstract：:Autoantibodies to myeloperoxidase (MPO) are a subset of anti-neutrophil cytoplasmic antibody (ANCA, MPO-ANCA) detected in the sera of some patients with primary systemic vasculitis. The titer of MPO-ANCA does not always reflect disease activity and this inconsistency may be attributable to differences in epitopic spec...

journal_title：Microbiology and immunology

authors： Suzuki K,Kobayashi S,Yamazaki K,Gondo M,Tomizawa K,Arimura Y,Nakabayashi K,Ozaki S,Yoshida M,Yoshida T,Tsusaka N,Muso E,Okazaki T,Hashimoto H

更新日期：2007-01-01 00:00:00

abstract：:Recurrent otitis media are frequently intractable during childhood. It is unclear whether recurrent otitis media is caused by etiological bacteria colonization or by new infections. Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis were isolated from the nasopharynx of 7 otitisprone and 2 non-...

journal_title：Microbiology and immunology

authors： Yokota S,Harimaya A,Sato K,Somekawa Y,Himi T,Fujii N

更新日期：2007-01-01 00:00:00